Structure of the human clamp loader bound to the sliding
Feb 18, 2020 · the clamp loader, which results in clamp closure around DNA and ejection of the clamp loader (Ason et al. 2003; Berdis and Benkovic 1996; Gomes et al. 2001; Jarvis et al. 1989). The sliding clamp is then successfully loaded at a primer-template junction for use by a DNA polymerase or other DNA metabolic enzymes.
Get a quote
Helicase Loading at Chromosomal Origins of Replication
How a DNA polymerase clamp loader opens a sliding clamp. Science 334 : 1675–1680 [ PMC free article ] [ PubMed ] [ Google Scholar ] Keyamura K, Fujikawa N, Ishida T, Ozaki S, Su'etsugu M, Fujimitsu K, Kagawa W, Yokoyama S, Kurumizaka H, Katayama T 2007.
Get a quote
(PDF) Cryo-EM structures reveal high-resolution mechanism
Sep 23, 2021 · Sliding clamps are ring-shaped protein complexes that are integral to the DNA replication machinery of all life. Sliding clamps are opened and installed onto DNA by clamp loader AAA+ ATPase complexes.
Get a quote
Differential effects of cisplatin and MNNG on dna mutants
DNA mismatch repair (MMR) in mammalian cells or Escherichia coli dam mutants increases the cytotoxic effects of cisplatin and N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). We found that, unlike wildtype, the dnaE486 (alpha catalytic subunit of DNA polymerase III holoenzyme) mutant, and a DnaX (clamp …
Get a quote
REVIEWS - Rockefeller University
machines known as CLAMP LOADERS,which crack open the ring and place it around the primed DNA in an ATP-driven reaction. Clamp loaders are composed of subunits that belong to the AAA+ family (BOX 1) and are conserved in bacteria,archaea and eukaryotes 11–14.The clamp loaders from each branch of life are likely to share a common
Get a quote
The Role of Replication Clamp-Loader Protein HolC of
In Escherichia coli, DNA replication is catalyzed by an assembly of proteins, the DNA polymerase III holoenzyme.This complex includes the polymerase and proofreading subunits, the processivity clamp, and clamp loader complex. The holC gene encodes an accessory protein (known as χ) to the core clamp loader complex and is the only protein of the holoenzyme that binds to single …
Get a quote
Allosteric communication in DNA polymerase clamp loaders
Apr 13, 2021 · Sliding DNA clamps and the ATP-driven clamp-loader complexes that load them onto DNA are essential components of the DNA replication machinery in all branches of life (Yao and O'Donnell, 2016).Sliding clamps, such as proliferating cell nuclear antigen (PCNA) in eukaryotes, are ring-shaped proteins that enable highly processive DNA replication by tethering …
Get a quote
Chemistry Tree - Brian A. Kelch
Kelch BA, Makino DL, O'Donnell M, et al. (2012) Clamp loader ATPases and the evolution of DNA replication machinery Bmc Biology. 10 Kelch B, Makino D, Simonetta K, et al. ( 2012 ) Molecular mechanisms of DNA polymerase clamp loaders Nato Science For Peace and Security Series a: Chemistry and Biology . 103-114
Get a quote
Brian A. Kelch - Publications
Clamp loader ATPases and the evolution of DNA replication machinery Bmc Biology. 10. PMID 22520345 DOI: 10.1186/1741-Liming : 1: 2012: Kelch B, Makino D, Simonetta K, O'Donnell M, Kuriyan J. Molecular mechanisms of DNA polymerase clamp loaders Nato Science For Peace and Security Series a: Chemistry and Biology. 103-114.
Get a quote
Sliding clamp-DNA interactions are required for viability
Jan 30, 2009 · In the case of beta(148-152), this defect was not due to altered interactions with the DnaX clamp loader, but rather was the result of impaired beta(148-152)-DNA interactions. Once loaded, beta(148-152) was proficient for DNA polymerase III (Pol III) replication in vitro.
Get a quote
UMass Medical School Kelch Lab BIochemistry & …
DNA Polymerase Clamp Loaders ; DNA Translocases ; Action of Enzymes on Sliding Clamps This is an official Page of the UMass Chan Medical School. Kelch Lab - Department of Biochemistry and Molecular Pharmacology • 364 Plantation St LRB923, Worcester MA 01605. Questions or Comments?
Get a quoteLet us know how access to this document benefits you.
UMass Chan Medical School the sliding clamp, the major polymerase processivity factor, and 2) the sliding clamp loader, a spiral-shaped AAA+ ATPase, which loads the clamp onto DNA. Before the clamp can act on DNA, the sliding clamp loader places the clamp onto DNA at primer/template (p/t) junctions. The clamp loader reaction
Get a quote
Structure of the SSB–DNA polymerase III interface and its
Structure of the SSB–DNA polymerase III interface and its role in DNA replication Aimee H Marceau1, Soon Bahng2,4, Shawn C Massoni3,4, Nicholas P George1, Steven J Sandler3,*, Kenneth J Marians2,* and James L Keck1,* 1Department of Biomolecular Chemistry, University of Wisconsin School of Medicine and Public Health, Madison, WI, USA, 2Molecular Biology …
Get a quote
DNA Polymerase Clamp Loaders - UMass Med
Chromosomal DNA replication requires that DNA polymerases be tethered to ring-shaped sliding clamps that encircle the DNA and allow for high-speed, processive replication. Sliding clamps are loaded onto DNA by the clamp loader complex, a pentameric assembly of proteins of the AAA+ family of ATPases. We want to understand the function and mechanism of clamp loaders in …
Get a quote
Kobelco Sk115sr-1es Hydraulic Crawler Excavator Workshop
This manual for Kobelco SK115SR-1ES Hydraulic Crawler Excavator is prepared to suite the needs of individuals who have basic knowledge in electrical and mechanical concepts. For those without basic knowledge, you should avoid performing complicated repairs to a vehicles equipment as it may render it unsafe.
Get a quote
Structure of the human clamp loader reveals an
Sep 09, 2020 · DNA replication and repair depend on AAA+ ATPase protein complexes called clamp loaders that open and load ring-shaped sliding clamps onto DNA. Using cryogenic electron microscopy, we determined the first structure of the human clamp loader (RFC), which is in an autoinhibited conformation while bound to the sliding clamp (PCNA). We assign this to be a …
Get a quote
The C-terminal Domain (CTD) of Human DNA Glycosylase NEIL1
Here, we show that NEIL1 binarily interacts with the proliferating cell nuclear antigen clamp loader replication factor C, DNA polymerase δ, and DNA ligase I in the absence of DNA via its non-conserved C-terminal domain (CTD); replication factor C interaction results in ∼8-fold stimulation of NEIL1 activity.
Get a quote
An Explanation for Lagging Strand Replication: Polymerase
complex clamp loader connected to Pol II1' (Maki et al., 1988; Onrust, 1993). The replicases of eukaryotes and phage T4 also utilize a sliding clamp protein and a clamp-loader complex for high processivity (Kuriyan and O'Donnell, 1993). The slid- ing …
Get a quote
Sliding clamp-DNA interactions are required for viability
Jan 30, 2009 · In the case of beta(148-152), this defect was not due to altered interactions with the DnaX clamp loader, but rather was the result of impaired beta(148-152)-DNA interactions. Once loaded, beta(148-152) was proficient for DNA polymerase III (Pol III) replication in vitro.
Get a quote
DNA clamp - Wikipedia
We present structures for the ATP-bound state of the clamp loader complex from bacteriophage T4, bound to an open clamp and primer-template DNA. The clamp loader traps a spiral conformation of the open clamp so that both the loader and the clamp match the helical symmetry of DNA. One structure reveals that ATP has been hydrolyzed in one subunit and …
Get a quote
